Does DNA Methylation Plays a Critical Role in Osteoblastic Differentiation of Mesenchymal Stem Cells (MSCs)?
نویسندگان
چکیده
1 Research Center of Thalassemia and Hemoglobinopathy, Jundishapur University of Medical Sciences, Ahvaz, IR Iran 2 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran 3 Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands 4 Young Researchers Club, Science and Research Branch, Islamic Azad University, Tehran, IR Iran
منابع مشابه
Evaluation of Changes in Global DNA Methylation during Osteoblastic Differentiation of Mesenchymal Stem Cells: A Laboratory Study
Background and Objectives: Control processes in osteoblastic differentiation of mesenchymal stem cells are not yet fully understood. Epigenetic mechanisms, especially the methylation of CpG Islands in the promoter of genes, are considered as one of the most important control mechanisms in stem cell differentiation. In the process of differentiation, it is debated whether only the methylation of...
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Background and Aim: Bone sialoprotein (BSP) is a specific marker of osteoblastic differentiation. In this research, the effect of Zoledronic Acid on BSP expression and methylation status during osteoblastic differentiation of mesenchymal stem cells (MSCs) was evaluated. Materials and Methods: In this experimental study, MSCs were isolated from human bone marrow. For osteogenic differentiation,...
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Background and Aim: Different regulation processes have an effect on osteoblastic differentiation of mesenchymal stem cells (MSCs), and among them Wnt signaling pathway is particularly desirable. In Wnt signaling pathway, Adenomatous Polyposis Coli (APC) bind to β-catenin and induce its degradation, thereby acting as a negative regulator of canonical Wnt pathway. In this study, gene expression ...
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Background and Objectives : RUNX2 is the most specific transcription factor in osteoblastic differentiation of MSCs. In this research, RUNX2 expression was quantified in MSCs differentiated by osteogenic differentiation medium (ODM) and zoledronic acid (ZA). Materials and Methods: In this experimental study, hMSCs were treated by osteogenic differentiation medium and ZA. RNA extraction was ...
متن کاملDifferent Methylation Patterns of RUNX2, OSX, DLX5 and BSP in Osteoblastic Differentiation of Mesenchymal Stem Cells
OBJECTIVE Runt-related transcription factor 2 (RUNX2) and osterix (OSX) as two specific osteoblast transcription factors and distal-less homeobox 5 (DLX5) as a non-specific one are of paramount importance in regulating osteoblast related genes including osteocalcin, bone sialoprotein (BSP), osteopontin and collagen type Iα1. The present study sets out to investigate whether epigenetic regulatio...
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